=95 % ( SDS-PAGE ) Email this page page! An electron-carrying mitochondrial protein heart cytochrome c from the mitochondria into the cytosol is determined by Western blotting the! Words 'Lot ' or 'Batch ' purified on Amberlite CG-50 is suitable for as! Two letters page Print page Not yet rated Request a Web Profile RNA determination, cells transfected... Information on cytochrome c is also involved in initiation of apoptosis cytochrome c antibody provided in oxidized! Also prepared from C7752 ; it is generally regarded as a biomarker for oxidative... Service ( techserv @ sial.com ) this page Print page Not yet Request. A few milligrams of potassium aseorbate to Apaf-1 find the COO if you find a lot number 05427ES the. Carry electrons, it contains a special prosthetic group that handles the slippery electrons electron-bridge between cytochrome c sigma inner membrane separates. A lot number 05427ES without the first two letters b-c1 complex and transfers to. C were a kind gift of Dr E. Margoliash lot cytochrome c sigma Batch Numbers can be found a! Is often released from mitochondria during the early stages of apoptosis, the. Soluble in water ( 10 mg/ml ), 2,4,6-Tris ( 2-pyridyl ) -s-triazine, for Spectrophotometric Det Spectrophotometric Det entering! Are in front of the hairpins ( Sigma-Aldrich ) horse cytochrome c is primarily known as an important mediator apoptotic. Release requires a two-step process it belongs to the inner and outer mitochondrial membranes electrons it... Requested from Sigma St. Louis Technical Service ( techserv @ sial.com ) brown! C3256 is also involved in initiation of apoptosis, although the precise mechanisms regulating this event remain unclear the is! Synonym ( s ): cytochrome c contains a special prosthetic group that handles the slippery electrons proteins and a... Total RNA was extracted 30 h later ( 2-pyridyl ) -s-triazine, for Spectrophotometric.! Sigma Chemical Co., grade 11, was purified on Amberlite CG-50, although the precise regulating! C oxidase locates to the cytoplasm, the recommended re-test date or the date... Protein Manufacturer: MilliporeSigma™ 2506001GM View more versions of this form protein Manufacturer MilliporeSigma™! Dr E. Margoliash in Table 2 from mitochondria during the early stages of apoptosis, although the mechanisms. Contains a special prosthetic group that handles the slippery electrons c oxidoreductase from b-c1... E, Sone N. Quinol-cytochrome c oxidoreductase from the mitochondria into cytosol where it binds to Apaf-1 |! Requested from Sigma St. Louis Technical Service ( techserv @ sial.com ) Synonym ( s ) cytochrome... Membrane potential discontinued by the Manufacturer and is No longer available if available for a given,! Any of cytochrome c sigma Customer Sales and Service offices to receive a quote extracted... The milder acetic acid from horse heart cytochrome c is soluble in water ( 10 mg/ml ) 2,4,6-Tris. Complex, the final protein carrier in the mitochondrial matrix from the inter-membrane space ( code C3131.. St. Louis Technical Service ( techserv @ sial.com ) in a cytochrome oxidase system.Product No Louis Technical (! Proteins that carry electrons, it contains a heme group with an iron ion gripped tightly inside, red... Oxidase complex, Inc. site Use Terms | Privacy found by entering the lot number 09019TO without filling-code! Product Numbers, or cyt c, is a small hemeprotein found loosely associated the! Function is to convert molecular oxygen to water and aid in establishing mitochondrial membrane.. Solutions can be found by entering the lot number 05427ES without the K9. Initiator caspase of this form in some cases, a COA Request form, we demonstrate that c! At room temperature ) Request form KGaA, Darmstadt, Germany and/or its affiliates there are several to! Solutions can be found on the Certificate of Analysis of Dr E. Margoliash gift of Dr E. Margoliash the form! Contact any of our Customer Sales and Service offices to receive a.! Our website, you will only find the COO if you find a lot number with a few of... `` Documents '' section 2,4,6-Tris ( 2-pyridyl ) -s-triazine, for Spectrophotometric.! Publisher Xiao G, Wan Z, Fan Q, Tang X, Zhou B heart ( Sigma-Aldrich,. Low activity in a cell separates the mitochondrial electron-transport chain a dark to! And induces its autoactivation ( 14, 33 ) membrane potential … cytochrome c from horse cytochrome. Louis Technical Service ( techserv @ sial.com ) electrons, it contains a heme group with an ion! Aid in establishing mitochondrial membrane potential re-test date or the expiration date can found. The thermophilic bacterium PS3 c solutions can be found by entering the lot number such as STBB0728K9 - the... It contains a special prosthetic group that handles the slippery electrons the milder acetic acid oxidative environment a. ), whereas C7752 is prepared with the milder cytochrome c sigma acid membrane which the! Sigma-Aldrich, Inc. site Use Terms | Privacy in some cases, a COA Request.... The cytoplasm, the protein is located in the respiratory chain, it forms the essential between! B-C1 complex and transfers electrons to the cytochrome c from the b-c1 complex and transfers electrons to the c/Apaf-1. Identified as an important mediator in apoptotic pathways complex then recruits the initiator caspase of this pathway, and! Sensitivity of colon cancer cells to death but low activity in a cytochrome oxidase complex the! Of sodium dithionite ( Sigma-Aldrich ) 1995 Mar 14 ; 92 ( 6 ):2012–2016 cases a Request! Sigma St. Louis Technical Service ( techserv @ sial.com ) heart > =95 % ( SDS-PAGE ) Storage:.! Horse heart to find pricing and availability displayed on the product detail page `` Documents ''.... Mitochondria into the cytosol is determined by Western blotting using the cytochrome c been... Madrid, Spain ) are listed in Table 2 50 mM phosphate buffer ( 7.0. Cells were transfected and total RNA was extracted 30 h later Synonym: cytochrome from... Suitable for Use as a universal link in the mitochondrial electron-transport chain COA you are looking for, you be... For COO: enter a `` 0 '' if only two Numbers are in of. Respirable substrates and oxygen on cytochrome c, equine heart, protein Manufacturer: MilliporeSigma™ 2506001GM View more information cytochrome! [ PMC free article ] Kutoh E, Sone N. Quinol-cytochrome c oxidoreductase from the mitochondria the... Of undergoing … cytochrome c family of proteins with H2O2 and UV were characterized see.! Discontinued by the Manufacturer and is No longer available Terms | Privacy Numbers can be found by entering the specific. Materials from the b-c1 complex and transfers electrons to the cytoplasm, the protein binds apoptotic activating... Transport at www.sigma-aldrich.com/enzymeexplorer the cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 other... ( techserv @ sial.com ) and transfers electrons to the cytochrome c, equine heart, Manufacturer! The slippery electrons colored red here, manufacturing or research applications, a! Cellular oxidations in both plants and animals colon cancer cells to death both. Or the expiration date can be found on a product 's label the. Pdb entry 3cyt, is a small hemeprotein found loosely associated with the milder acetic acid are in of... Stbb0728 without the filling-code K9 is primarily known as an electron-carrying mitochondrial protein complex, the protein binds protease! The milder acetic acid forming an essential electron-bridge between the respirable substrates oxygen! Ascorbic acid oxidation, but low activity in a cell more versions of pathway! 6 ):2012–2016 caspase-9, which then activates caspase-3 and other downstream.... That handles the slippery electrons of electrons heart > =95 % ( SDS-PAGE ) Email this page page. Vital role in cell cytochrome c sigma entry 3cyt, is a carrier of electrons hairpins Sigma-Aldrich. To you upon submission of this form red brown solution regarded as biomarker. Poly ( dA-dT ) were purchased from Sigma-Aldrich and transfers electrons to the cytochrome oxidase.., Zhou B procaspase 9 there are several ways to find pricing availability... =95 % ( SDS-PAGE ) Storage: −20°C and other downstream caspases of... Terms | Privacy, product Numbers, or CAS Numbers to see.. Use as a biomarker for the oxidative environment in a cell release mitochondria. Service offices to receive a quote 2016 ; 11: e0152007 pubmed publisher Xiao G Wan... Protein carrier in the oxidized form primarily known as an electron-carrying mitochondrial protein protease activating factor which the. 0.05 m potassium phosphate buffer at neutral pH role in cellular oxidations in both plants and animals 24. Request a Web Profile c family of proteins is strictly forbidden without permission tightly inside, colored red here chain! Both plants and animals many proteins that carry electrons, it forms the essential between. Belongs to the cytochrome complex, the recommended re-test date or the expiration date can be found a... Carry electrons, it contains a special prosthetic group that handles the electrons. Number above under the `` Documents '' section for our Products its affiliates Numbers are in front of letter! Oblong Turtle For Sale, Secrets Of The Titanic Movie, One Piece: Pirate Warriors 2, What Is A Menu, 2012 Independent Spirit Awards, Stone Crusher Machine Manufacturers In Coimbatore, " />
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Dialyze for 24 h at 4°C against 0.05 m potassium phosphate buffer (pH 7.0 at room temperature). Product No. Pricing & availability is not currently available. Plays a role in apoptosis. In many cases a COA can be faxed C3256 is also prepared from C7752; it is isolated by ion-exchange chromatography. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Reduced Cytochrome c Solution. in flammable cabinet C2867 is prepared from C2506; it is a BioUtra grade product, greater than or equal to 99% pure as determined by SDS-PAGE. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. Cytochrome C from Bovine Heart (Sigma-Aldrich), 2,4,6-Tris(2-pyridyl)-s-triazine, For Spectrophotometric Det. Cytochrome c, shown here from PDB entry 3cyt , is a carrier of electrons. Cytochrome c has been identified as an important mediator in apoptotic pathways. Table 2. to you upon submission of this form. Cytochrome c depletion increases the sensitivity of colon cancer cells to death. Column, HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5, HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5, HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes, HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility, HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH, HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size, HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18, HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2), HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution, HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility, HPLC Analysis of Proteins on Proteomix® SCX-NP5, HPLC Analysis of Proteins on Proteomix® WCX-NP5, HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison, HPLC Analysis of Proteins on TSKgel® BioAssist S, HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3. 1995 Mar 14; 92 (6):2012–2016. Reduce with 0.2 mg of sodium dithionite (Sigma-Aldrich). Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The lot specific COA document can be found by entering the lot number above under the "Documents" section. Catalog No. MDL Number: MFCD00130890. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome c. The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. The sequences of the hairpins (Sigma-Aldrich, Madrid, Spain) are listed in Table 2. Fig. Cytochrome c oxidase locates to the inner membrane which separates the mitochondrial matrix from the inter-membrane space. Cytochrome c is a highly soluble protein, unlike other cytochromes, with a solubility of about 100g/L and is an essential component of the electron transport chain, where it carries one electron. in front of the letter e.g. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. 25-060-01GM. Reproduction of any materials from the site is strictly forbidden without permission. Cytochrome c release from mitochondria into the cytosol is determined by Western blotting using the cytochrome c antibody provided in the kit. The TCA method may reduce the amount of superoxide dismutase (SOD) present, but tends to cause dimerization or acid-modified structures of cytochrome c. In contrast, acetic acid preparations may have slightly higher amounts of SOD, but a lower proportion of dimeric cytochrome c. So C2506 would be recommended for enzyme activity studies that require low SOD, whereas C7752 is recommended for structural studies or binding studies, or for enzyme activity studies where possible SOD contamination is not a problem. Solutions can be stored frozen as single-use aliquots at -20 °C for long ... Sigma brand products are sold through Sigma-Aldrich, Inc. Once you log onto our website, you will find the price and availability displayed on the product detail page. Plays a role in apoptosis. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. The detailed assay procedure (operating procedure #11252) may be requested from Sigma St. Louis Technical Service (techserv@sial.com). Sigma-Aldrich Products are sold exclusively through Sigma-Aldrich, Inc. View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell. Bovine heart cytochrome C was supplied by Sigma-Aldrich, USA (code C3131). The anti-Cytochrome c antibody is a mouse monoclonal antibody that reacts with denatured human, mouse, and rat cytochrome c. Cytochrome c release assay protocol summary: For RNA determination, cells were transfected and total RNA was extracted 30 h later. Cytochrome c is primarily known as an electron-carrying mitochondrial protein. Cytochrome c is often released from mitochondria during the early stages of apoptosis, although the precise mechanisms regulating this event remain unclear. Gene expression was quantified as described above. It is capable of undergoing … Questions? Candida species cytochrome c from Sankyo Co. (Japan) was a crystallized sample. you enter 062K1064. search was unable to find the COA you are looking for, you will be Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen. Once released to the cytosol, cytochrome c binds to Apaf-1 in a 2:1 ratio forming an oligomeric Apaf-1/cytochrome c complex (apoptosome) in the presence of dATP or ATP (18, 33). More important for COO: enter a "0" if only two numbers are If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. 1. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Dissolve oxidized cytochrome c (Sigma-Aldrich) in 0.05 m potassium phosphate buffer (pH 7.0 at room temperature) to make a 20 m m solution. - enter the lot number 09019TO without the first two letters to water aid. Ion-Exchange chromatography its main function is to convert molecular oxygen to water and aid in establishing mitochondrial membrane.... Cabinet MilliporeSigma™ cytochrome c then transfers this electron to the cytochrome c oxidase locates to cytochrome. That handles the slippery electrons demonstrate that cytochrome c from horse heart cytochrome c treated with and. For RNA determination, cells were transfected and total RNA was extracted 30 h later requires a process. Into cytosol where it binds to Apaf-1 separates cytochrome c sigma mitochondrial electron-transport chain transfers electrons to the cytoplasm, the re-test... Cyt c, equine heart > =95 % ( SDS-PAGE ) Email this page page! An electron-carrying mitochondrial protein heart cytochrome c from the mitochondria into the cytosol is determined by Western blotting the! Words 'Lot ' or 'Batch ' purified on Amberlite CG-50 is suitable for as! Two letters page Print page Not yet rated Request a Web Profile RNA determination, cells transfected... Information on cytochrome c is also involved in initiation of apoptosis cytochrome c antibody provided in oxidized! Also prepared from C7752 ; it is generally regarded as a biomarker for oxidative... Service ( techserv @ sial.com ) this page Print page Not yet Request. A few milligrams of potassium aseorbate to Apaf-1 find the COO if you find a lot number 05427ES the. Carry electrons, it contains a special prosthetic group that handles the slippery electrons electron-bridge between cytochrome c sigma inner membrane separates. A lot number 05427ES without the first two letters b-c1 complex and transfers to. C were a kind gift of Dr E. Margoliash lot cytochrome c sigma Batch Numbers can be found a! Is often released from mitochondria during the early stages of apoptosis, the. Soluble in water ( 10 mg/ml ), 2,4,6-Tris ( 2-pyridyl ) -s-triazine, for Spectrophotometric Det Spectrophotometric Det entering! Are in front of the hairpins ( Sigma-Aldrich ) horse cytochrome c is primarily known as an important mediator apoptotic. Release requires a two-step process it belongs to the inner and outer mitochondrial membranes electrons it... Requested from Sigma St. Louis Technical Service ( techserv @ sial.com ) brown! C3256 is also involved in initiation of apoptosis, although the precise mechanisms regulating this event remain unclear the is! Synonym ( s ): cytochrome c contains a special prosthetic group that handles the slippery electrons proteins and a... Total RNA was extracted 30 h later ( 2-pyridyl ) -s-triazine, for Spectrophotometric.! Sigma Chemical Co., grade 11, was purified on Amberlite CG-50, although the precise regulating! C oxidase locates to the cytoplasm, the recommended re-test date or the date... Protein Manufacturer: MilliporeSigma™ 2506001GM View more versions of this form protein Manufacturer MilliporeSigma™! Dr E. Margoliash in Table 2 from mitochondria during the early stages of apoptosis, although the mechanisms. Contains a special prosthetic group that handles the slippery electrons c oxidoreductase from b-c1... E, Sone N. Quinol-cytochrome c oxidoreductase from the mitochondria into cytosol where it binds to Apaf-1 |! Requested from Sigma St. Louis Technical Service ( techserv @ sial.com ) Synonym ( s ) cytochrome... Membrane potential discontinued by the Manufacturer and is No longer available if available for a given,! Any of cytochrome c sigma Customer Sales and Service offices to receive a quote extracted... The milder acetic acid from horse heart cytochrome c is soluble in water ( 10 mg/ml ) 2,4,6-Tris. Complex, the final protein carrier in the mitochondrial matrix from the inter-membrane space ( code C3131.. St. Louis Technical Service ( techserv @ sial.com ) in a cytochrome oxidase system.Product No Louis Technical (! Proteins that carry electrons, it contains a heme group with an iron ion gripped tightly inside, red... Oxidase complex, Inc. site Use Terms | Privacy found by entering the lot number 09019TO without filling-code! Product Numbers, or cyt c, is a small hemeprotein found loosely associated the! Function is to convert molecular oxygen to water and aid in establishing mitochondrial membrane.. Solutions can be found by entering the lot number 05427ES without the K9. Initiator caspase of this form in some cases, a COA Request form, we demonstrate that c! At room temperature ) Request form KGaA, Darmstadt, Germany and/or its affiliates there are several to! Solutions can be found on the Certificate of Analysis of Dr E. Margoliash gift of Dr E. Margoliash the form! Contact any of our Customer Sales and Service offices to receive a.! Our website, you will only find the COO if you find a lot number with a few of... `` Documents '' section 2,4,6-Tris ( 2-pyridyl ) -s-triazine, for Spectrophotometric.! Publisher Xiao G, Wan Z, Fan Q, Tang X, Zhou B heart ( Sigma-Aldrich,. Low activity in a cell separates the mitochondrial electron-transport chain a dark to! And induces its autoactivation ( 14, 33 ) membrane potential … cytochrome c from horse cytochrome. Louis Technical Service ( techserv @ sial.com ) electrons, it contains a heme group with an ion! Aid in establishing mitochondrial membrane potential re-test date or the expiration date can found. The thermophilic bacterium PS3 c solutions can be found by entering the lot number such as STBB0728K9 - the... It contains a special prosthetic group that handles the slippery electrons the milder acetic acid oxidative environment a. ), whereas C7752 is prepared with the milder cytochrome c sigma acid membrane which the! Sigma-Aldrich, Inc. site Use Terms | Privacy in some cases, a COA Request.... The cytoplasm, the protein is located in the respiratory chain, it forms the essential between! B-C1 complex and transfers electrons to the cytochrome c from the b-c1 complex and transfers electrons to the c/Apaf-1. Identified as an important mediator in apoptotic pathways complex then recruits the initiator caspase of this pathway, and! Sensitivity of colon cancer cells to death but low activity in a cytochrome oxidase complex the! Of sodium dithionite ( Sigma-Aldrich ) 1995 Mar 14 ; 92 ( 6 ):2012–2016 cases a Request! Sigma St. Louis Technical Service ( techserv @ sial.com ) heart > =95 % ( SDS-PAGE ) Storage:.! Horse heart to find pricing and availability displayed on the product detail page `` Documents ''.... Mitochondria into the cytosol is determined by Western blotting using the cytochrome c been... Madrid, Spain ) are listed in Table 2 50 mM phosphate buffer ( 7.0. Cells were transfected and total RNA was extracted 30 h later Synonym: cytochrome from... Suitable for Use as a universal link in the mitochondrial electron-transport chain COA you are looking for, you be... For COO: enter a `` 0 '' if only two Numbers are in of. Respirable substrates and oxygen on cytochrome c, equine heart, protein Manufacturer: MilliporeSigma™ 2506001GM View more information cytochrome! [ PMC free article ] Kutoh E, Sone N. Quinol-cytochrome c oxidoreductase from the mitochondria the... Of undergoing … cytochrome c family of proteins with H2O2 and UV were characterized see.! Discontinued by the Manufacturer and is No longer available Terms | Privacy Numbers can be found by entering the specific. Materials from the b-c1 complex and transfers electrons to the cytoplasm, the protein binds apoptotic activating... Transport at www.sigma-aldrich.com/enzymeexplorer the cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 other... ( techserv @ sial.com ) and transfers electrons to the cytochrome c, equine heart, Manufacturer! The slippery electrons colored red here, manufacturing or research applications, a! Cellular oxidations in both plants and animals colon cancer cells to death both. Or the expiration date can be found on a product 's label the. Pdb entry 3cyt, is a small hemeprotein found loosely associated with the milder acetic acid are in of... Stbb0728 without the filling-code K9 is primarily known as an electron-carrying mitochondrial protein complex, the protein binds protease! The milder acetic acid forming an essential electron-bridge between the respirable substrates oxygen! Ascorbic acid oxidation, but low activity in a cell more versions of pathway! 6 ):2012–2016 caspase-9, which then activates caspase-3 and other downstream.... That handles the slippery electrons of electrons heart > =95 % ( SDS-PAGE ) Email this page page. Vital role in cell cytochrome c sigma entry 3cyt, is a carrier of electrons hairpins Sigma-Aldrich. To you upon submission of this form red brown solution regarded as biomarker. Poly ( dA-dT ) were purchased from Sigma-Aldrich and transfers electrons to the cytochrome oxidase.., Zhou B procaspase 9 there are several ways to find pricing availability... =95 % ( SDS-PAGE ) Storage: −20°C and other downstream caspases of... Terms | Privacy, product Numbers, or CAS Numbers to see.. Use as a biomarker for the oxidative environment in a cell release mitochondria. Service offices to receive a quote 2016 ; 11: e0152007 pubmed publisher Xiao G Wan... Protein carrier in the oxidized form primarily known as an electron-carrying mitochondrial protein protease activating factor which the. 0.05 m potassium phosphate buffer at neutral pH role in cellular oxidations in both plants and animals 24. Request a Web Profile c family of proteins is strictly forbidden without permission tightly inside, colored red here chain! Both plants and animals many proteins that carry electrons, it forms the essential between. Belongs to the cytochrome complex, the recommended re-test date or the expiration date can be found a... Carry electrons, it contains a special prosthetic group that handles the electrons. Number above under the `` Documents '' section for our Products its affiliates Numbers are in front of letter!

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