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Optimization of the Zebrafish Embryo Developmental Toxicity Assay (ZEDTA) M.Tobor-Kaplon, D. van den Oetelaar, M. Beekhuijzen, H. Emmen, E. van de Waart Charles River, Den Bosch, The Netherlands 1 Introduction The ZEDTA is a promising and innovative method with a potential to replace the screening of teratogenicity in animals exposure on zebrafish embryo through acute toxicity assay assessment. Expose zebrafish embryos to at least 1,000 chemicals. Materials and Methods. 2. ( 2015 ) performed high content screening of zebrafish embryos to examine the developmental toxicity of ethanol, nicotine, ketamine, and caffeine. New methodologies of genome … Zebrafish embryos exhibit unique characteristics, including ease of maintenance and drug administration, short reproductive cycle, and transparency that permits visual assessment of developing cells and organs. Correlation between zebrafish embryo log LC 50 and rodent log LD 50. The toxic effects of these plant extracts were compared using in vitro cytotoxicity assay using 1.1B4 (human-derived pancreatic β-cell line), 3T3-L1 (mouse-derived adipocyte like cells), and WRL-68 cell (human hepatic cell line) types. Fertilized embryos (Tropical 5D) were selected and staged, according to Kimmel et al. The protocol deals with exposing zebrafish embryos to a range of compound concentrations at 28°C throughout organogenesis, i.e. Zebrafish (Danio rerio) has been a prominent model vertebrate in a variety of biological disciplines.Substantial information gathered from developmental and genetic research, together with near-completion of the zebrafish genome project, has placed zebrafish in an attractive position for use as a toxicological model. To examine the ability of zebrafish assays to predict toxicity in rodents, we analysed a correlation between our zebrafish embryo log LC 50 values, and rodent log LD 50 from the literature. In view of safety of pregnant women, a promising in vitro zebrafish embryo developmental toxicity assay has been developed to test pharmaceutical and chemical compounds for their teratogenic potential. Toxicity Assays 2.3.1. Using a 1000 L wide-bore micropipette tip, embryos were The rates of morphological changes are one type of endpoints used to generate dose response curves. . 2.3. Image‐based high content assays for toxicity screening using transgenic zebrafish embryos with morphology and behavioral endpoints are also gaining popularity. Lantz‐McPeak et al. Abstract. 1.1. Thus, the aim of this study was to evaluate the effects of aqueous Momordica charantia Linn. The … Abstract. Because of these advantages, zebrafish bioassays are cheaper and faster than mouse assays, and are suitable for large-scale drug screening. 2) Study the morphology and behavior of the embryos to look for evidence of developmental toxicity. Embryonic and larval Danio rerio (zebrafish) is increasingly used as a toxicological model to conduct rapid in vivo tests and developmental toxicity assays; the zebrafish features high genetic homology to mammals, robust, phenotypes, high-throughput genetic and chemical screening have made it a powerful tool to evaluate in vivo toxicity. Zebrafish Embryonic Toxicity (ZET) Assay Fertilized zebrafish embryos were transferred to a new polystyrene disposable petri dish and rinsed in HEPES-bu ered E3 (HE3) media (5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2-2H2O, 0.33 mM MgSO4-7H2O, 10 mM HEPES, pH 7.2). Thus, out of all plant’s the whole plant extract of OS was found as the lowest toxic to zebrafish embryos . Zebrafish Embryo Model. The zebrafish embryo toxicity test presented here is based on a 24 h exposure of 4, 24 and 96 h post fertilization (hpf) embryos in a static system. 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